5N62
Human TTR crystals soaked in manganese chloride.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-26 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.891993 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 43.270, 85.830, 63.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.638 - 1.800 |
R-factor | 0.1976 |
Rwork | 0.195 |
R-free | 0.24450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5k1j |
RMSD bond length | 0.009 |
RMSD bond angle | 1.025 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.176 | 2.175 |
Number of reflections | 42596 | 3134 |
<I/σ(I)> | 11.53 | 1.52 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.37 | 7.61 |
CC(1/2) | 0.998 | 0.390 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | protein: 10 mg/ml Dialysed in 100 milli-M NaCl, 50 milli-M sodium acetate, pH 5.5 precipitant: 26% polyethylene glycol 4,000 (PEG4K), 0.16 M imidazole malate, pH 6.0 cryosoak: 40 % CM7 (12.5 % di-ethylene glycol + 12.5 % ethylene glycol + 12.5 % glycerol + 25 % 1,2-propanediol + 12.5 % DMSO), 25 % MPEG 5K, 5 mM MnCl2, 10 min soak. |