5N4F
Prolyl oligopeptidase B from Galerina marginata - apo protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-16 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 90.890, 105.620, 86.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.000 - 2.400 |
R-factor | 0.2176 |
Rwork | 0.216 |
R-free | 0.25374 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.171 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 54.000 |
High resolution limit [Å] | 2.400 |
Number of reflections | 31438 |
<I/σ(I)> | 14.5 |
Completeness [%] | 98.6 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 30% PEG4000 and 100 mM MES buffer, pH 6.5. |