5N41
Archaeal DNA polymerase holoenzyme - SSO6202 at 1.35 Ang resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-10 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97949 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 61.752, 61.752, 35.363 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.876 - 1.351 |
R-factor | 0.1622 |
Rwork | 0.160 |
R-free | 0.19810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5n35 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.954 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575-000)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.900 | 1.400 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.046 | 0.339 |
Number of reflections | 16515 | 1178 |
<I/σ(I)> | 28.2 | 3.1 |
Completeness [%] | 95.2 | 68.6 |
Redundancy | 4.8 | 3 |
CC(1/2) | 0.839 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294.15 | OD280=17.7 mg/ml in 20 mM HEPES pH 7.5, 0.3 M NaCl, 1 mM MgCl2 and 1 mM b-ME mixed with 0.1 M Potassium thiocyanate and 30% w/v Polyethylene glycol monomethyl ether 2000 (Hampton Index) |