5N30

Crystal structure of the V72I mutant of the mouse alpha-Dystroglycan N-terminal region

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-10
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9763
Spacegroup name	H 3
Unit cell lengths	71.810, 71.810, 143.990
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.905 - 1.800
R-factor	0.1555
Rwork	0.154
R-free	0.18240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u2c
RMSD bond length	0.016
RMSD bond angle	1.338
Data reduction software	XDS (VERSION Oct 15, 2015)
Data scaling software	XSCALE (VERSION Oct 15, 2015 BUILT=20151231)
Phasing software	PHASER (2.7.2)
Refinement software	PHENIX ((1.11_2561: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.105	1.306
Rmeas	0.106	1.362
Number of reflections	25641	2530
<I/σ(I)>	15.84	2.13
Completeness [%]	99.9	99.4
Redundancy	11.5	11.3
CC(1/2)	0.999	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	277	Cross streak-Seeding (from D109N alpha-DG mutant) 1 muL protein solution + 1 muL precipitant Seeding after 3-6 days of equilibration Protein Solution: (5 mg / mL protein, 25 mM Tris, 150 mM NaCl, pH 7.5) Precipitant: 0.8 M citrate buffer, pH 7.2