5MZR
X-ray structure of the H235Q mutant of GLIC in complex with propofol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-07-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 182.842, 132.989, 161.162 |
| Unit cell angles | 90.00, 102.83, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.650 |
| R-factor | 0.1922 |
| Rwork | 0.191 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.650 |
| Number of reflections | 108419 |
| <I/σ(I)> | 9.8 |
| Completeness [%] | 99.0 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 100 mM NaAct pH4 400 mM NaSCN 16% glycerol 2% DMSO 12-15% PEG4K |
