Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2011-02-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.95367 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 127.211, 168.839, 98.495 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.948 - 3.048 |
| R-factor | 0.1954 |
| Rwork | 0.193 |
| R-free | 0.23270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | homology model based on 1CU1 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.713 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.950 | 3.210 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.091 | 0.482 |
| Number of reflections | 40242 | |
| <I/σ(I)> | 15.2 | 1.8 |
| Completeness [%] | 98.3 | 93 |
| Redundancy | 5.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 293 | 12% PEG 3350 250 mM Tri-sodium citrate 100 mM 2-aminoethanesulfonic acid |
