5MYQ
Crystal structure of avidin in complex with ferrocene homobiotin derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91840 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.933, 82.081, 74.601 |
| Unit cell angles | 90.00, 106.29, 90.00 |
Refinement procedure
| Resolution | 71.600 - 1.890 |
| R-factor | 0.18711 |
| Rwork | 0.186 |
| R-free | 0.23201 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vyo |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.185 |
| Data reduction software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.600 | |
| High resolution limit [Å] | 1.890 | |
| Rmerge | 0.052 | 0.423 |
| Number of reflections | 42387 | |
| <I/σ(I)> | 9.36 | 1.97 |
| Completeness [%] | 97.1 | 94.7 |
| Redundancy | 1.92 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292.15 | 0.2 M ammonium iodide, 22 % w/v PEG 3350 |
