5MYI
Convergent evolution involving dimeric and trimeric dUTPases in signalling.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97948 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 79.450, 79.450, 125.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.367 - 1.900 |
| R-factor | 0.1672 |
| Rwork | 0.165 |
| R-free | 0.20870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.924 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.810 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.040 | 0.390 |
| Number of reflections | 19182 | 2723 |
| <I/σ(I)> | 11.1 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.3 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 296 | 30% PEG400 0.2 M MgCl2, 0.1 M Hepes 7.5 |
