5MX5
Mouse PA28alpha-beta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 126.090, 137.380, 136.420 |
| Unit cell angles | 90.00, 104.44, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.900 |
| R-factor | 0.24453 |
| Rwork | 0.243 |
| R-free | 0.27226 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5msj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.926 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.059 | 0.532 |
| Number of reflections | 96305 | |
| <I/σ(I)> | 12.1 | 2.3 |
| Completeness [%] | 96.4 | 92.8 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.2 M KH2PO4, 9 % PEG3350 |
