5MW7

Human Jagged2 C2-EGF3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.92
Spacegroup name	P 21 21 21
Unit cell lengths	46.814, 77.205, 96.357
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.251 - 2.800
R-factor	0.2579
Rwork	0.255
R-free	0.31210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cc1
RMSD bond length	0.004
RMSD bond angle	0.662
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.200	2.970
High resolution limit [Å]	2.800	2.800
Rmerge	0.267	1.116
Number of reflections	8975
<I/σ(I)>	6.2	1.5
Completeness [%]	99.4	99.7
Redundancy	4.8	5
CC(1/2)	0.977	0.648

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.3	293	PEG-5000 MME, Sodium citrate