5MW5

Human Jagged2 C2-EGF2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-04
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.3
Spacegroup name	P 21 21 21
Unit cell lengths	48.266, 83.878, 99.352
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.091 - 2.700
R-factor	0.2315
Rwork	0.230
R-free	0.26350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mv7
RMSD bond length	0.006
RMSD bond angle	0.686
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.900	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.107	0.559
Number of reflections	11513
<I/σ(I)>	12.9	3.7
Completeness [%]	99.6	100
Redundancy	7.7	8.1
CC(1/2)	0.996	0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	PEG 8000, potassium dihydrogen phosphate