5MVK
Crystal structure of an unmodified A-DNA dodecamer containing 3 consecutive CpG steps
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.970 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 43.678, 43.678, 58.887 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.826 - 1.531 |
| R-factor | 0.1809 |
| Rwork | 0.180 |
| R-free | 0.18770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.379 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.830 |
| High resolution limit [Å] | 1.530 |
| Number of reflections | 10206 |
| <I/σ(I)> | 19.7 |
| Completeness [%] | 99.9 |
| Redundancy | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Sodium chloride (12 mM) Potassium chloride (80 mM) Sodium cacodylate (40 mM, pH 7.0) Spermine tetrahydrochloride (12 mM) MPD (40% v/v) |
