5MVF

Active structure of EHD4 complexed with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918409
Spacegroup name	P 42 21 2
Unit cell lengths	199.469, 199.469, 41.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.378 - 3.268
R-factor	0.239
Rwork	0.238
R-free	0.26440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cid
RMSD bond length	0.002
RMSD bond angle	0.563
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.380	3.460
High resolution limit [Å]	3.268	3.270
Rmerge	0.274
Rmeas		2.115
Rpim	0.088
Number of reflections	13647
<I/σ(I)>	9.53	1.02
Completeness [%]	99.5	97
Redundancy	10.5
CC(1/2)	0.995	0.361

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	900 mM sodium malonate (pH 7)