5MV8
Structure of human Myosin 7b C-terminal MyTH4-FERM domain in complex with harmonin-a PDZ3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.906032 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.620, 42.560, 118.170 |
| Unit cell angles | 90.00, 98.02, 90.00 |
Refinement procedure
| Resolution | 35.330 - 1.880 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.880 | |
| Number of reflections | 53567 | |
| <I/σ(I)> | 12.77 | 1 |
| Completeness [%] | 95.0 | 61 |
| Redundancy | 6.4 | |
| CC(1/2) | 0.998 | 0.543 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 7% (w/v) PEG 4000, 0.1 M HEPES pH 7.5, 50 mM MgCl2 |
