5MV0

Structure of an N-terminal domain of a reptarenavirus L protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	56.970, 112.342, 153.848
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	90.730 - 1.930
R-factor	0.175
Rwork	0.173
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.865
Data reduction software	iMOSFLM (7.1.3)
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.730	2.000
High resolution limit [Å]	1.931	1.931
Number of reflections	74927	7396
<I/σ(I)>	15.33
Completeness [%]	100.0	100
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		279	20% PEG 200, 2.5% PEG 3000, and 100 mM MES, pH 5.7