5MUM
Glycoside Hydrolase BACINT_00347
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 53.313, 68.438, 118.696 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.600 - 1.800 |
| R-factor | 0.15353 |
| Rwork | 0.152 |
| R-free | 0.19096 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4irt |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.554 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 68.600 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 255841 |
| <I/σ(I)> | 12 |
| Completeness [%] | 98.4 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG3350 and 200 mM Tri-potassium Citrate |
