5MUF
Crystal structure of human phosphoglycerate mutase family member 5 (PGAM5) in its enzymatically active dodecameric form induced by the presence of the N-terminal WDPNWD motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-04-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97630 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 82.215, 141.403, 183.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.030 - 3.100 |
| R-factor | 0.22793 |
| Rwork | 0.226 |
| R-free | 0.25553 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mxo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.104 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.030 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.271 | 0.993 |
| Rpim | 0.101 | 0.373 |
| Number of reflections | 18243 | 2709 |
| <I/σ(I)> | 6.8 | 2 |
| Completeness [%] | 94.3 | 96.2 |
| Redundancy | 7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 293 | 18% PEG 3350 and 0.1 M MES, pH 5.7 |
