5MUE

Self-assembled alpha-Tocopherol Transfer Protein Nanoparticles Promote Vitamin E Delivery Across an Endothelial Barrier

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-31
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.99987
Spacegroup name	I 4 3 2
Unit cell lengths	168.184, 168.184, 168.184
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.641 - 2.397
R-factor	0.184
Rwork	0.182
R-free	0.21520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oip
RMSD bond length	0.002
RMSD bond angle	0.617
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.551	48.551	2.540
High resolution limit [Å]	2.397	7.120	2.400
Rmerge	0.091	0.023	1.543
Rmeas	0.095	0.025	1.621
Number of reflections	30125	700	2543
<I/σ(I)>	20.8	71.31	1.54
Completeness [%]	99.7	97.8	99.1
Redundancy	10.744	9.097	10.692
CC(1/2)	0.999	1.000	0.599

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	291.15	0.1 M Ammonium sulfate,10% w/v Polyethylene glycol 4,000