5MU2
ACC1 Fab fragment in complex with CII583-591 (CG10)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-16 |
| Detector | PILATUS 6 |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 148.861, 156.008, 156.592 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.090 - 2.700 |
| R-factor | 0.20737 |
| Rwork | 0.206 |
| R-free | 0.22845 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mu0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.484 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.090 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.121 | 0.799 |
| Number of reflections | 100498 | 4899 |
| <I/σ(I)> | 12.1 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.2 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% (w/v) PEG3350, 0.1 M Bistris-Propane pH 7.5, 0.2 M sodium acetate |
