5MU1
UDP-Glucose Glycoprotein Glucosyltransferase from Chaetomium thermophilum soaked with K2PtI6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-16 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.07141 |
| Spacegroup name | P 61 |
| Unit cell lengths | 116.950, 116.950, 301.491 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 101.280 - 3.480 |
| R-factor | 0.238 |
| Rwork | 0.237 |
| R-free | 0.25200 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.240 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | autoSHARP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 150.750 | 3.670 |
| High resolution limit [Å] | 3.480 | 3.480 |
| Rmerge | 3.573 | |
| Rmeas | 3.730 | |
| Rpim | 1.089 | |
| Number of reflections | 28757 | 4354 |
| <I/σ(I)> | 15.4 | 1.3 |
| Completeness [%] | 96.2 | 100 |
| Redundancy | 23.2 | 23.3 |
| CC(1/2) | 0.482 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 51.4 mM MES pH 3.3, 44.6 Imidazole pH 8.3, 20% PEG 8K, 10% Ethylene Glycole, 120 mM Morpheus Ethylene glycols mix |
