5MTW
Mycobacterium tuberculosis Rv1957 SecB-like chaperone in complex with a ChAD peptide from Rv1956 HigA1 antitoxin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.072522 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.860, 89.960, 91.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.090 - 1.840 |
R-factor | 0.2144 |
Rwork | 0.212 |
R-free | 0.26488 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fx3 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.941 |
Data reduction software | XDS (autoPROC 1.1.7) |
Data scaling software | XSCALE (autoPROC 1.1.7) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.090 | 1.867 |
High resolution limit [Å] | 1.835 | 1.835 |
Rmerge | 0.093 | 0.796 |
Number of reflections | 63188 | 3135 |
<I/σ(I)> | 13.7 | 2.3 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 6.5 | 6.7 |
CC(1/2) | 0.999 | 0.338 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 285 | 25-27 % PEG 1500, calcium acetate 80-240 mM, 10 % DMSO, 100 mM Tris pH 8.0 |