5MTV

Active structure of EHD4 complexed with ATP-gamma-S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918409
Spacegroup name	P 42 21 2
Unit cell lengths	199.973, 199.973, 41.537
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.500 - 2.790
R-factor	0.2278
Rwork	0.227
R-free	0.24310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cid
RMSD bond length	0.011
RMSD bond angle	0.785
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	2.960
High resolution limit [Å]	2.790	2.790
Rmerge	0.095	0.910
Rpim	0.035
Number of reflections	21733	3412
<I/σ(I)>	19.04	2.15
Completeness [%]	99.8	99.2
Redundancy	7.2	7.65
CC(1/2)	0.999	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Sodium polyacrylate 5100, magnesium chloride, HEPES