5MTM

Monobody Mb(Lck_3) bound to Lck-SH2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	81.834, 81.834, 105.961
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.917 - 2.405
R-factor	0.2078
Rwork	0.205
R-free	0.25680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.159
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.920
High resolution limit [Å]	2.400
Number of reflections	14544
<I/σ(I)>	37.91
Completeness [%]	100.0
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1M MES pH6.5, 15 % PEG 20000