5MTD
Crystal structure of PDF from the Vibrio parahaemolyticus bacteriophage VP16T - crystal form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.980110 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.260, 64.260, 125.690 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.670 - 1.500 |
| R-factor | 0.18782 |
| Rwork | 0.187 |
| R-free | 0.20957 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lry |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.311 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 48767 | 3520 |
| <I/σ(I)> | 17.35 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4.32 | 4.36 |
| CC(1/2) | 0.999 | 0.778 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 292 | 28% PEG-1000 100mM sodium acetate |
