5MSN
Structure of the Dcc1 Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2014-07-31 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9200 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.682, 100.697, 83.495 |
| Unit cell angles | 90.00, 100.67, 90.00 |
Refinement procedure
| Resolution | 29.357 - 2.002 |
| R-factor | 0.1912 |
| Rwork | 0.189 |
| R-free | 0.23010 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.592 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.360 | 2.074 |
| High resolution limit [Å] | 2.000 | 2.002 |
| Rmerge | 0.116 | 0.974 |
| Number of reflections | 71317 | |
| <I/σ(I)> | 8.43 | 1.31 |
| Completeness [%] | 95.0 | 87 |
| Redundancy | 4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M potassium sodium tartrate and 18% PEG 3,350 |
