5MSM
Structure of the Dcc1-Ctf8-Ctf18C Trimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2015-07-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.610, 164.220, 60.640 |
| Unit cell angles | 90.00, 90.55, 90.00 |
Refinement procedure
| Resolution | 60.640 - 2.290 |
| R-factor | 0.2029 |
| Rwork | 0.200 |
| R-free | 0.25980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5msn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.531 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.640 | 2.372 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.085 | 0.456 |
| Number of reflections | 46232 | |
| <I/σ(I)> | 7.15 | 1.31 |
| Completeness [%] | 90.0 | 57 |
| Redundancy | 3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Bis-Tris Propane pH 6.3, 0.2M NaBr and 17% PEG 3,350 |
