5MSE
GFP nuclear transport receptor mimic 3B8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.401, 73.398, 93.927 |
| Unit cell angles | 90.00, 93.73, 90.00 |
Refinement procedure
| Resolution | 46.860 - 1.660 |
| R-factor | 0.17532 |
| Rwork | 0.174 |
| R-free | 0.20573 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ema |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.318 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.280 | 1.723 |
| High resolution limit [Å] | 1.664 | 1.664 |
| Rmerge | 0.129 | 1.910 |
| Number of reflections | 114871 | |
| <I/σ(I)> | 12.47 | 1.18 |
| Completeness [%] | 98.4 | 96.49 |
| Redundancy | 6.8 | 7 |
| CC(1/2) | 0.998 | 0.468 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 293 | 5-9% PEG8000, 28% glycerol, 100?mM Imidazole (pH 7.1) |
