5MS6
High-salt structure of RavZ LIR2-fused human LC3B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.99992 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 69.300, 69.300, 117.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.000 - 1.900 |
R-factor | 0.2082 |
Rwork | 0.206 |
R-free | 0.26090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z0d |
RMSD bond length | 0.008 |
RMSD bond angle | 1.169 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.000 | 1.968 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.203 | 1.551 |
Number of reflections | 23355 | |
<I/σ(I)> | 14.37 | 1.52 |
Completeness [%] | 100.0 | 100 |
Redundancy | 23.9 | 15.2 |
CC(1/2) | 0.999 | 0.203 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 0.1 M sodium acetate and 3 M NaCl |