5MRX

27-nt SRL with a 5-hydroxymethyl cytidine modification

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-27
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.77
Spacegroup name	P 43
Unit cell lengths	29.510, 29.510, 76.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.510 - 0.851
R-factor	0.1587
Rwork	0.145
R-free	0.16820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dvz
RMSD bond length	0.006
RMSD bond angle	1.123
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	0.850
Rmeas	0.045
Number of reflections	57106
<I/σ(I)>	30.32
Completeness [%]	99.6
Redundancy	22.36

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	well: 3.0M (NH4)2SO4 drop: 50mM KMops pH7.0, 10mM MnCl2, 10mM MgCl2, 1.0mM Na-EDTA pH 8.0, 10mM Tris pH 8.0, RNA 2.5mg/ml 2vol drop + 1vol well