5MRT

Crystal structure of L5 protease Lysobacter sp. XL1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-27
Detector	Bruker Platinum 135
Wavelength(s)	1.54179
Spacegroup name	P 21 21 21
Unit cell lengths	62.549, 66.668, 96.642
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.334 - 1.600
R-factor	0.1704
Rwork	0.168
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mrr
RMSD bond length	0.006
RMSD bond angle	0.911
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	1.700
High resolution limit [Å]	1.600	1.600
Number of reflections	53331
<I/σ(I)>	9.7	1.8
Completeness [%]	98.6	97.7
Redundancy	5.9	3.46

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	2,7M Na formate, 0,2 M ammonium acetate, PIPES