5MQZ
Archaeal branched-chain amino acid aminotransferase from Archaeoglobus fulgidus; holoform
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9200 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.920, 139.120, 167.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.860 - 2.100 |
| R-factor | 0.1829 |
| Rwork | 0.181 |
| R-free | 0.21797 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eiy |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.781 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.560 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.135 | 2.270 |
| Number of reflections | 99985 | |
| <I/σ(I)> | 10 | 0.8 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 6.3 | 5.4 |
| CC(1/2) | 0.997 | 0.292 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 291 | 50 % PEG 200,0.2 M Magnesium chloride hexahydrate, 0.1 M Sodium cacodylate |
