5MQQ

Transcriptional repressor AmtR of corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-04
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	P 21 21 2
Unit cell lengths	149.783, 160.638, 51.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.240 - 2.093
R-factor	0.2112
Rwork	0.209
R-free	0.24970
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.939
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.240	2.220
High resolution limit [Å]	2.090	2.090
Rmerge	0.086	0.751
Number of reflections	73411
<I/σ(I)>	13.5	2.6
Completeness [%]	98.8	95.9
Redundancy	4.05	3.86

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	293	AmtR (12 mg/ml) in 20 mM Tris HCl, pH 8.0, 0.2 M NaCl Crystals obtained under the conditions: 0.1 M Bis-Tris pH 5.8, 20 % PEG 3350 and 0.2 M (NH4)2SO4 with a protein to drop ratio of 1:1 microliters