5MPO
Crystal structure of human molybdopterin synthase complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 65 |
| Unit cell lengths | 123.720, 123.720, 82.490 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.860 - 2.430 |
| R-factor | 0.20718 |
| Rwork | 0.205 |
| R-free | 0.25860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2Q5W:D 4AP8:A |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.765 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.860 | 2.490 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.062 | 0.955 |
| Rpim | 0.028 | 0.472 |
| Number of reflections | 27171 | |
| <I/σ(I)> | 15.8 | 1.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.8 | 6.1 |
| CC(1/2) | 0.635 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | 12% PEG3350 -- 0.1M potassium nitrate -- 0.05M potassium thiocyanate |
