5MP8
Crystal Structure of CK2alpha with ZT0432 bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-25 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.8726 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 65.170, 68.340, 331.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.160 - 1.920 |
| R-factor | 0.213 |
| Rwork | 0.212 |
| R-free | 0.22430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cvh |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.160 | 1.989 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.031 | 0.560 |
| Number of reflections | 52615 | |
| <I/σ(I)> | 12.6 | 1.1 |
| Completeness [%] | 92.0 | 88 |
| Redundancy | 1.9 | 1.8 |
| CC(1/2) | 0.999 | 0.615 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 112.5mM Mes pH 6.5, 35% glycerol ethoxylate, 180 mM ammonium acetate |
