5MOH
Crystal structure of CK2alpha with ZT0583 bound.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-14 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 0.9600 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.663, 45.659, 63.833 |
| Unit cell angles | 90.00, 111.70, 90.00 |
Refinement procedure
| Resolution | 59.310 - 1.380 |
| R-factor | 0.1969 |
| Rwork | 0.197 |
| R-free | 0.20260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cvh |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS ((VERSION January 10) |
| Data scaling software | Aimless (0.2.14) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.310 | 1.390 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.045 | 0.385 |
| Number of reflections | 58088 | |
| <I/σ(I)> | 13.9 | 2.1 |
| Completeness [%] | 90.5 | 45.5 |
| Redundancy | 3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 107mM Mes pH 6.5, 29% glycerol ethoxylate, 1 M ammonium acetate |
