5MOG
Oryza sativa phytoene desaturase inhibited by norflurazon
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.00003 |
Spacegroup name | I 4 |
Unit cell lengths | 230.210, 230.210, 179.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.250 - 2.770 |
R-factor | 0.2095 |
Rwork | 0.209 |
R-free | 0.22440 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.000 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.250 | 2.820 |
High resolution limit [Å] | 2.770 | 2.770 |
Rmerge | 0.165 | |
Number of reflections | 118342 | |
<I/σ(I)> | 14.2 | 1.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14 | 14.3 |
CC(1/2) | 0.999 | 0.533 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 24 % (w/v) PEG5000 MME, 0.1 M HEPES/NaOH pH 7.0, 2 % (v/v) Tacsimate pH 7.0 |