5MOG
Oryza sativa phytoene desaturase inhibited by norflurazon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.00003 |
| Spacegroup name | I 4 |
| Unit cell lengths | 230.210, 230.210, 179.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.250 - 2.770 |
| R-factor | 0.2095 |
| Rwork | 0.209 |
| R-free | 0.22440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.250 | 2.820 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rmerge | 0.165 | |
| Number of reflections | 118342 | |
| <I/σ(I)> | 14.2 | 1.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14 | 14.3 |
| CC(1/2) | 0.999 | 0.533 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 24 % (w/v) PEG5000 MME, 0.1 M HEPES/NaOH pH 7.0, 2 % (v/v) Tacsimate pH 7.0 |
