5MNV
Structural and functional characterization of OleP in complex with 6DEB in PEG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-30 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.918007 |
| Spacegroup name | P 1 |
| Unit cell lengths | 112.500, 116.700, 123.300 |
| Unit cell angles | 103.90, 104.30, 114.70 |
Refinement procedure
| Resolution | 39.970 - 2.970 |
| R-factor | 0.242 |
| Rwork | 0.238 |
| R-free | 0.31100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.206 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 121.000 | 3.170 |
| High resolution limit [Å] | 2.970 | 2.970 |
| Rmerge | 0.091 | 0.486 |
| Number of reflections | 101917 | |
| <I/σ(I)> | 11.92 | 2.2 |
| Completeness [%] | 97.5 | 91.8 |
| Redundancy | 3.49 | 3.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 0,4M NaCl, 10mM TRIS, 22% PEG3350 |
