5MN7
S. aureus FtsZ 12-316 F138A GTP Closed form (3FCm)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-03 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9282 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 116.091, 130.015, 134.125 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.677 - 3.300 |
| R-factor | 0.2132 |
| Rwork | 0.211 |
| R-free | 0.26810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mn5 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.505 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.098 | 0.719 |
| Number of reflections | 12453 | |
| <I/σ(I)> | 11.6 | 1.9 |
| Completeness [%] | 91.5 | 94.3 |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.696 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | 10 mg/ml; 40% PEG monomethyl ether 350, 0.05 M MES pH 6 |
