5MMY

Crystal structure of OXA10 with HEPES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	48.772, 102.189, 127.409
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.410 - 1.880
Rwork	0.178
R-free	0.19790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fq9
Data reduction software	XDS
Data scaling software	Aimless (0.5.17)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	79.720	79.720	1.930
High resolution limit [Å]	1.880	8.410	1.880
Rmerge	0.176	0.064	2.092
Rmeas	0.183
Rpim	0.051
Total number of observations	679292
Number of reflections	52703
<I/σ(I)>	11.8	23.7	3.2
Completeness [%]	100.0	100	99.8
Redundancy	12.9	11.5	12.9
CC(1/2)	0.997	0.998	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.1M HEPES pH 7.0, 20% PEG 6000