5MLA
Crystal structure of human RAS in complex with darpin K55
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | H 3 |
| Unit cell lengths | 112.830, 112.830, 66.770 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.410 - 2.190 |
| R-factor | 0.18407 |
| Rwork | 0.182 |
| R-free | 0.22892 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.612 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.410 | 2.250 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.074 | 0.716 |
| Number of reflections | 15629 | |
| <I/σ(I)> | 12.8 | 1.3 |
| Completeness [%] | 95.7 | 74.4 |
| Redundancy | 4.6 | 2.5 |
| CC(1/2) | 0.998 | 0.477 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 18% PEG8000, 200mM Calcium Acetate, 100mM Sodium Cacodylate pH6.5 |
