5ML7

CRYSTAL STRUCTURE OF L1-STALK FRAGMENT OF 23S rRNA FROM HALOARCULA MARISMORTUI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-27
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.8266
Spacegroup name	P 41 21 2
Unit cell lengths	136.836, 136.836, 70.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.300
R-factor	0.2291
Rwork	0.226
R-free	0.29141
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3u4m
RMSD bond length	0.008
RMSD bond angle	1.720
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.380
High resolution limit [Å]	3.190	3.190
Number of reflections	10777
<I/σ(I)>	7.97	3.96
Completeness [%]	93.3	83.7
Redundancy	5.3	4.81
CC(1/2)		0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	291	20% MPD, 0.1M Magnesium Acetate, 0.05M MES