5MKV
Crystal Structure of Human Dihydropyrimidinease-like 2 (DPYSL2A)/Collapsin Response Mediator Protein (CRMP2) residues 13-516
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-04 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.920 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.900, 157.510, 87.420 |
| Unit cell angles | 90.00, 93.61, 90.00 |
Refinement procedure
| Resolution | 19.870 - 1.800 |
| R-factor | 0.1682 |
| Rwork | 0.167 |
| R-free | 0.19963 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gse |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.952 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.870 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 194435 |
| <I/σ(I)> | 15.84 |
| Completeness [%] | 97.7 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.2M ammonium acetate, 30% PEG 4000 and 0.1M citrate buffer pH 5.5 |
