5MKT
Crystal structure of mouse prorenin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 41 |
| Unit cell lengths | 141.232, 141.232, 82.961 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.210 - 3.200 |
| R-factor | 0.26499 |
| Rwork | 0.263 |
| R-free | 0.29723 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4amt |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.012 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.250 | 3.420 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.088 | 0.739 |
| Number of reflections | 71150 | |
| <I/σ(I)> | 9.5 | 1.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.2 | 5.6 |
| CC(1/2) | 0.998 | 0.827 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 0.75 K/Na H2PO4, 0.1M HEPES PH 7.9 |
