5MKN

Crystal structure of SmAP (LSm) protein from Methanococcus vannielii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.972420
Spacegroup name	P 1 21 1
Unit cell lengths	91.015, 97.869, 123.448
Unit cell angles	90.00, 103.88, 90.00

Refinement procedure

Resolution	48.935 - 2.550
R-factor	0.2366
Rwork	0.233
R-free	0.31470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xq3
RMSD bond length	0.005
RMSD bond angle	0.890
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.650
High resolution limit [Å]	2.550	2.550
Rmerge		0.480
Number of reflections	68169
<I/σ(I)>	12.27	2.4
Completeness [%]	98.7	99.7
Redundancy	3.75	3.84
CC(1/2)		0.914

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	30% PEG 400, 50 mM Tris HCl pH 8.5, 100 mM KCl, 10 mM MgCl2, 15 mM UMP