5MJP

Multi-bunch pink beam serial crystallography: Phycocyanin (One chip)

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	293
Detector technology	CCD
Collection date	2016-07-10
Detector	RAYONIX MX340-HS
Wavelength(s)	1.15-1.25
Spacegroup name	H 3 2
Unit cell lengths	187.800, 187.800, 60.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.900 - 2.110
R-factor	0.172
Rwork	0.169
R-free	0.20000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jbo
RMSD bond length	0.003
RMSD bond angle	0.828
Data reduction software	Precognition
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	14.920	2.220
High resolution limit [Å]	2.106	2.120
Rmerge	0.061
Number of reflections	15458	9048
<I/σ(I)>	31.68
Completeness [%]	64.7	26.6
Redundancy	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	Ammonium sulphate