5MJM

Single-shot pink beam serial crystallography: Phycocyanin (Five chips merged)

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	293
Detector technology	CCD
Collection date	2016-07-09
Detector	RAYONIX MX340-HS
Wavelength(s)	1.15-1.25
Spacegroup name	H 3 2
Unit cell lengths	187.800, 187.800, 60.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.917 - 2.302
R-factor	0.1428
Rwork	0.139
R-free	0.18100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jbo
RMSD bond length	0.005
RMSD bond angle	0.903
Data reduction software	Epinorm
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	14.920	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.057
Number of reflections	12168
<I/σ(I)>	47.2
Completeness [%]	68.5	0.23
Redundancy	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		293	Ammonium sulphate