5MIK
X-ray structure of carboplatin-encapsulated horse spleen apoferritin (rotating anode data)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-10 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 180.654, 180.654, 180.654 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 104.300 - 1.960 |
| R-factor | 0.14847 |
| Rwork | 0.146 |
| R-free | 0.19161 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5erk |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.729 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.300 | 2.000 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.107 | 0.489 |
| Number of reflections | 18700 | |
| <I/σ(I)> | 6.2 | 3.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.9 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 298 | 0.8 M (NH4)2SO4, 0.1 M Tris pH 7.7, 60 mM CdSO4. |
