5MIJ
X-ray structure of carboplatin-encapsulated horse spleen apoferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-28 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.670, 181.670, 181.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 104.890 - 1.490 |
| R-factor | 0.15886 |
| Rwork | 0.158 |
| R-free | 0.18101 |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.180 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.900 | 1.520 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.084 | 0.429 |
| Number of reflections | 42236 | |
| <I/σ(I)> | 14.8 | 4.2 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 298 | 0.8 M (NH4)2SO4, 0.1 M Tris pH 7.7, 60 mM CdSO4. |
