5MHG
Crystal structure of anti-T4 Fab fragment with tetraiodoBPA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.767, 56.992, 61.993 |
| Unit cell angles | 90.00, 98.39, 90.00 |
Refinement procedure
| Resolution | 20.090 - 2.801 |
| R-factor | 0.2245 |
| Rwork | 0.221 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mhe |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.353 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.090 | 2.970 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 11247 | |
| <I/σ(I)> | 6.7 | 1.21 |
| Completeness [%] | 98.8 | 95.5 |
| Redundancy | 5 | 4.9 |
| CC(1/2) | 0.530 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2 M Ammonium sulfate, 25 % PEG 3350, 0.1 M 3350 |
