5MH2
Crystal structure of a DM9 domain containing protein from Crassostrea gigas with D22A mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.99999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.160, 56.530, 105.530 |
| Unit cell angles | 90.00, 93.41, 90.00 |
Refinement procedure
| Resolution | 19.600 - 1.300 |
| R-factor | 0.147 |
| Rwork | 0.145 |
| R-free | 0.18200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mh0 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.577 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 106083 | |
| <I/σ(I)> | 17.4 | 2.5 |
| Completeness [%] | 98.9 | 96.1 |
| Redundancy | 6.3 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M HEPES, 30% PEG 3350, 0.12M Magnisium Chloride hexahydrate |
